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Účinky vybraných peptidů na jednobuněčnou řasu Chlamydomonas reinhardtii
Avdeeva, Olga
In the presented work, the modified novicidin peptides NVCAA and NVCHH were applied to the unicellular alga Chlamydomonas reinhardtii. The aim of the study was to determine the minimum inhibitory concentrations of the tested peptides on Escherichia coli bacteria with subsequent application on the unicellular algae Chlamydomonas reinhardtii. The assessment of the effect of the tested peptides on the algae was carried out using spectrophotometric analyses: chlorophyll A and chlorophyll B content, total flavonoid content, total antioxidant capacity and using the DPPH ASSAY method. Chlamydomonas reinhardtii molecular changes were determined by monitoring the amount of isolated RNA and the expression of APX1, APX2, CAT1, CAT2 genes using the Real-time PCR method. Statistically processed data showed that the application of NVCAA and NVCHH peptides induced considerable stress in the cilia.
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Quantitation of transcript bcr / abl in chronic myeloid leukemia
PYTLOVÁ, Kamila
This bachelor thesis in the theoretical part deals with the disease chronic myelogenous leukemia (CML), which accounts for about 25% of the leukemia of the adult age, with maximum incidence in the age group between 50.-70. year of life. With low frequency occurs even in children and adolescents. Chronic myeloid leukemia is characterized by the presence of the hybrid gene bcr/abl, that arises as a result of reciprocal translocation t(9;22)(q34;q11) karyotypicaly detectable as a Ph chromosome. The gene bcr/abl is the hallmark of CML used to refine the diagnosis and monitoring of treatment success. Quantification is carried out either as a relative (often), when the amount of fluorescence of the test gene is compared with the amount of fluorescence of the reference gene, or absolute, when the amount of DNA we are going deduct from the calibration curve, which will provide us with a DNA sample of a given concentration. The aim of this work is to compare different ways of quantification of the transcript bcr/abl. The pursuit of quantification of this transcript has been going on for a long time, involved here organization from around the world. The most sensitive method for detection of the presence of this aberration is the reverse transcription polymerase chain reaction (RT-PCR) detecting the transcript bcr/abl. The second objective of this work is to give an overview of this issue and perform one of the methods for the relative quantification of the gene bcr/abl. The final objective is to get acquainted with the management of samples in the laboratory, and verification and validation of these methods.
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Optimization of miRNA analysis in fine-needle biopsy samples of pancreatic cancer tissue.
Čuperková, Romana ; Benešová, Lucie (advisor) ; Kuthan, Martin (referee)
Pancreatic cancer (PC) is extremely severe malignant disease with a five-year survival of less than 5%. Currently there is no reliable tool for the diagnosis of PC in its early stages. At the time of clinical symptoms most patients are in an advanced stage of the disease and the treatment does not usually have a significant effect. For these reasons emphasis is gradually shifting to the search for the suitable molecular markers for improvement of the diagnosis and assessment of the survival prognosis with respect to a possibility of surgical treatment. MiRNA represent one of the most promising markers, although, their examination in pancreatic tissue is a complicated process. One of the reasons is the very small amount of the source material coming from a fine needle biopsy. A second cause of problems is the subtle character of the pancreatic tissue resulting in significantly lower yields of molecular genetic analysis when compared to other epithelial tissues. An additional negative factor is heterogeneity of the tissue resulting in disproportionate representation of tumor cells within the sample. A suitable choice of procedures for isolation of nucleic acids (NA) and subsequent analysis including quantification of tumor cells is critical for accurate evaluation of the miRNA levels. This work is...
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Sharka (Plum pox virus) of stone fruits
JAKUBÍKOVÁ, Hedvika
Plum pox virus causes one of the most devastating stone fruit diseases. The main target of this thesis is a detection of Plum pox virus in infected leaf tissue of Prunus domestica.. The presence of the virus in a plant material was confirmed by the RNA isolation and its use as a template for RT-PCR. Furthermore, the basic information about properties of the virus, common methods of the virus detection and protection possibilities against the virus is are included.
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